ATP hydrolysis is a biological process in which adenosine triphosphate (ATP) molecules are broken down into adenosine diphosphate (ADP) and inorganic phosphate (Pi), releasing energy in the process. This energy released during ATP hydrolysis is often utilized by cells to drive various cellular processes such as muscle contraction, ion transport, and biosynthetic reactions. The hydrolysis of ATP is catalyzed by enzymes known as ATPases, which play a crucial role in cellular metabolism and energy transduction. Research in the field of ATP hydrolysis aims to understand the mechanisms and regulation of this essential cellular process, as well as its implications in various physiological and pathological conditions.